LOCUS       Exported                4002 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0050_producer_luxI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Daniel Gerngross
  TITLE     piG0050
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        50..84
                     /note="const. promoter (J23100)"
     RBS             93..104
                     /note="RBS (B0034)"
     CDS             111..695
                     /codon_start=1
                     /note="luxI (C0161)"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*"
     terminator      707..793
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      908..1679
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1680..2342
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2353..2461
                     /note="incP origin of transfer"
     CDS             complement(2607..3797)
                     /note="TetR"
     terminator      3904..3998
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag
       61 ctcagtccta ggtacagtgc tagctactag tgaaagagga gaaatactag atgactataa
      121 tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc
      181 ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg
      241 aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa
      301 atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt
      361 ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc
      421 gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga
      481 aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag
      541 taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg
      601 gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta
      661 atgaacagtt taaaaaagca gtcttaaatt aataaattaa aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      781 gagtaggaca aatccgccgc cctagacagc tgggcgcgcc ccccctacgg gcttgctctc
      841 cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc ccgtggatat gtggacgatg
      901 gccgcgagcg gccaccggct ggctcgcttc gctcggcccg tggacaaccc tgctggacaa
      961 gctgatggac aggctgcgcc tgcccacgag cttgaccaca gggattgccc accggctacc
     1021 cagccttcga ccacataccc accggctcca actgcgcggc ctgcggcctt gccccatcaa
     1081 tttttttaat tttctctggg gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg
     1141 gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc
     1201 ttgacgcgcc tggaacgacc caagcctatg cgagtggggg cagtcgaagg gcgaagcccg
     1261 cccgcctgcc ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac
     1321 cttgggcaag gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc
     1381 cggaggggga gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca
     1441 ccaaagaact agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg
     1501 ggggtacgca acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa
     1561 aatctgtaat tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt
     1621 tgcagctgat tgcgcatggt gccgcaaccg tgcggcaccc ctaccgcatg gagataagca
     1681 tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt cactgggtgc
     1741 aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg
     1801 caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc
     1861 agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac gcagtcaagg
     1921 acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc accgtgtcgg
     1981 cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg
     2041 tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg ttggggcatg
     2101 gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg accggccccg
     2161 gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg ccagccttga
     2221 ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc gatgagccgt
     2281 gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtaacgctg ccgcgccggt
     2341 agggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     2401 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     2461 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     2521 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     2581 ttatgcagcg gaaaaggaca attgtctcag gtcgaggtgg cccggctcca tgcaccgcga
     2641 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
     2701 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt
     2761 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
     2821 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     2881 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
     2941 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     3001 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     3061 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     3121 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg ccacaatggt catgccccgc
     3181 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggacg gcgctctccc
     3241 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     3301 gccgcaagga atggtgcgtg cagggagatg gcgcccaaca gtcccccggc cacggggcct
     3361 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     3421 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     3481 gccacgatgc gtccggcgta gagaatccac aggacgggtg tggtcgccat gatcgcgtag
     3541 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
     3601 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
     3661 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
     3721 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     3781 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
     3841 ccgcattaca gtttatcgat gataagctgt caaatttaaa tcgtaattat tggggacccc
     3901 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     3961 gagttctgag gtcattactg gatctatcaa caggagtcca ag
//
